PriMicro Database

For classification, identification and molecular phylogenetic analyses of microbes

Primer Detail

Primer name
fRPB2-7cR
Gene name
RNA polymerase II (RPB2)
Primer sequence (5'-3')
CCCAT(A/G)GCTTG(T/C)TT(A/G)CCCAT
Direction
R
Category
fungus-specific
Target
fungi
Reference
Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16: 1799-1808.
Literature
Valérie Reeb, François Lutzoni and Claude Roux (2004) Contribution of RPB2 to multilocus phylogenetic studies of the euascomycetes (Pezizomycotina, Fungi) with special emphasis on the lichen-forming Acarosporaceae and evolution of polyspory. Molecular Phylogenetics and Evolution 32: 1036-1060.
EWA Boehm, GK Mugambi, AN Miller, SM Huhndorf, S Marincowitz, JW Spatafora and CL Schoch (2009) A molecular phylogenetic reappraisal of the Hysteriaceae, Mytilinidiaceae and Gloniaceae (Pleosporomycetidae, Dothideomycetes) with keys to world species. Studies in Mycology 64: 49-83.
M Réblová, W Gams and KA Seifert (2011) Monilochaetes and allied genera of the Glomerellales, and a reconsideration of families in the Microascales. Studies in Mycology 68: 163-191.
WM Jaklitsch and H Voglmayr (2015) Biodiversity of Trichoderma (Hypocreaceae) in Southern Europe and Macaronesia. Studies in Mycology 80: 1-87.
Priscila Chaverri and Gary J Samuels (2003) Hypocrea/Trichoderma (Ascomycota, Hypocreales, Hypocreaceae): species with green ascospores. Studies in Mycology 48: 1-116.
Reference Strain
Trichoderma aeroaquaticumNBRC 108031, NBRC 108034