PriMicro Database

Primer Detail

Primer name

fRPB2-5F
FRPB2-5f
f5F

Gene name

RNA polymerase II second largest subunit (RPB2)

Primer sequence (5'-3')

GAYGAYMGWGATCAYTTYGG

Direction

F

Category

fungus-specific

Target

fungi

Reference

Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16: 1799-1808.

Literature

Valérie Reeb, François Lutzoni and Claude Roux (2004) Contribution of RPB2 to multilocus phylogenetic studies of the euascomycetes (Pezizomycotina, Fungi) with special emphasis on the lichen-forming Acarosporaceae and evolution of polyspory. Molecular Phylogenetics and Evolution 32: 1036-1060.

EWA Boehm, GK Mugambi, AN Miller, SM Huhndorf, S Marincowitz, JW Spatafora and CL Schoch (2009) A molecular phylogenetic reappraisal of the Hysteriaceae, Mytilinidiaceae and Gloniaceae (Pleosporomycetidae, Dothideomycetes) with keys to world species. Studies in Mycology 64: 49-83.

Tazick Z and Tajick Ghanbary MA (2013) Taxonomic position of Bipolaris oryzae among other Cochliobolus species using Ribosomal region and some Protein Coding genes. Research Journal of Recent Sciences 2: 212-216.

Conrad L. Schocha, Keith A. Seifertb, Sabine Huhndorfc, Vincent Robertd, John L. Spougea, C. André Levesqueb, Wen Chenb and Fungal Barcoding Consortium (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences

Houbraken J and Samson RA (2011) Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Studies in Mycology 70: 1-51.

M Réblová, W Gams and KA Seifert (2011) Monilochaetes and allied genera of the Glomerellales, and a reconsideration of families in the Microascales. Studies in Mycology 68: 163-191.

P. Brandon Matheny (2005) Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe; Agaricales). Molecular Phylogenetics and Evolution 35: 1-20.

Verkley GJ, Quaedvlieg W, Shin HD and Crous PW (2013) A new approach to species delimitation in Septoria. Studies in Mycology 75: 213-305.

Quaedvlieg W, Verkley GJ, Shin HD, Barreto RW, Alfenas AC, Swart WJ, Groenewald JZ and Crous PW (2013) Sizing up Septoria. Studies in Mycology 75: 307-390.

Quaedvlieg W, Groenewald JZ, Jesús Yáñez-Morales M and Crous PW (2012) DNA barcoding of Mycosphaerella species of quarantine importance to Europe. Persoonia 29: 101-115.

Stukenbrock EH, Quaedvlieg W, Javan-Nikhah M, Zala M, Crous PW, (2012) Zymoseptoria ardabilia and Z. pseudotritici, two progenitor species of the septoria tritici leaf blotch fungus Z. tritici (synonym: Mycosphaerella graminicola). Mycologia 104: 1397-1407.

Quaedvlieg W, Kema GHJ, Groenewald JZ, Verkley GJM, Seifbarghi S, Razavi M, Gohari A, Mirzadi, Mehrabi R and Crous PW (2011) Zymoseptoria gen. nov.: a new genus to accommodate Septoria-like species occurring on graminicolous hosts. Persoonia 26: 57-69.

Bischoff JF, Rehner SA and Humber RA (2006) Metarhizium frigidum sp. nov.: a cryptic species of M. anisopliae and a member of the M. flavoviride complex. Mycologia 98: 737-745.

Matheny PB, Wang Z, Binder M, Curtis JM, Lim YW, Nilsson RH, Hughes KW, Hofstetter V, Ammirati JF, Schoch CL, Langer E, Langer G, McLaughlin DJ, Wilson AW, Frøslev T, Ge ZW, Kerrigan RW, Slot JC, Yang ZL, Baroni TJ, Fischer M, Hosaka K, Matsuura K, Seidl MT, Vauras J and Hibbett DS (2007) Contributions of rpb2 and tef1 to the phylogeny of mushrooms and allies (Basidiomycota, Fungi). Molecular Phylogenetics and Evolution 43: 430-451.

X-Z Liu, Q-M Wang, B Theelen, M Groenewald, F-Y Bai and T Boekhout (2015) Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses. Studies in Mycology 81: 1-26.

Q-M Wang, M Groenewald, M Takashima, B Theelen, P-J Han, X-Z Liu, T Boekhout and F-Y Bai (2015) Phylogeny of yeasts and related filamentous fungi within Pucciniomycotina determined from multigene sequence analyses. Studies in Mycology 81: 27-53.

Q-M Wang, B Theelen, M Groenewald, F-Y Bai and T Boekhout (2014) Moniliellomycetes and Malasseziomycetes, two new classes in Ustilaginomycotina. Persoonia 33: 41-47.

WM Jaklitsch and H Voglmayr (2015) Biodiversity of Trichoderma (Hypocreaceae) in Southern Europe and Macaronesia. Studies in Mycology 80: 1-87.

Walter M Jaklitsch, Monika Komon, Christian P Kubicek and Irina S Druzhinina (2005) Hypocrea voglmayrii sp. nov. from the Austrian Alps represents a new phylogenetic clade in Hypocrea/Trichoderma. Mycologia 97: 1365-1378.

Priscila Chaverri and Gary J Samuels (2003) Hypocrea/Trichoderma (Ascomycota, Hypocreales, Hypocreaceae): species with green ascospores. Studies in Mycology 48: 1-116.

Reference Strain

Aspergillus aculeatus	IFM 47778, IMI 211388, JCM 22898
Aspergillus amylovorus	IMI 129961
Aspergillus arenarius	IMI 055632
Aspergillus avenaceus	IFM 49793, IMI 016140, JCM 2719, NBRC 7539, NBRC 8129
Aspergillus biplanus	IMI 235602
Aspergillus brunneouniseriatus	IMI 227677
Aspergillus candidus	IMI 091889
Aspergillus cervinus	IMI 107684, JCM 1722
Aspergillus clavatoflavus	IFM 49795, IMI 124937
Aspergillus clavatus	IMI 15949
Aspergillus conjunctus	IMI 135421
Aspergillus egyptiacus	IMI 141415
Aspergillus funiculosus	IFM 50946, IMI 054397, JCM 2352, NBRC 8131
Aspergillus janus	IMI 016065
Aspergillus kanagawaensis	IMI 126690, JCM 1723, NBRC 6219
Aspergillus ochraceus	IMI 016247, JCM 1958
Aspergillus penicilliformis	IMI 129968
Aspergillus pulvinus	IMI 139628
Aspergillus restrictus	IMI 016267, JCM 1727
Aspergillus sparsus	IMI 019394, JCM 2357
Aspergillus versicolor	IMI 45554, NBRC 30338
Aspergillus wentii	IMI 017295
Aspergillus zonatus	IFM 50940, IMI 124936, JCM 2254, NBRC 8817
Bannoa hahajimensis	JCM 10336
Basipetospora halophilica	IFO 9650
Bensingtonia ingoldii	JCM 7445, NBRC 10578
Bensingtonia miscanthi	JCM 5733, NBRC 10579
Bensingtonia musae	IFM 49877, JCM 8801, NBRC 102559
Bensingtonia naganoensis	JCM 5978, NBRC 10580
Bensingtonia phyllada	JCM 7476, NBRC 10581
Bensingtonia sakaguchii	JCM 10047, NBRC 102558
Bensingtonia subrosea	IFM 57755, JCM 5735, NBRC 10582
Bensingtonia thailandica	JCM 10651, NBRC 102560
Bensingtonia yuccicola	IFM 49223, JCM 6251, NBRC 10596
Chrysosporium inops	IMI 096729, NBRC 7583
Curvibasidium cygneicollum	IFM 48566, IFM 48595, JCM 9029, JCM 10310, NBRC 100752
Eupenicillium hirayamae	IFM 48426, IMI 78255, JCM 22785, NBRC 6435
Eurotium amstelodami	IFM 47794, IMI 229971, JCM 1565, JCM 10260, NBRC 33018
Eurotium herbariorum	IMI 211383, JCM 1575
Fennellia flavipes	IFM 40894, IMI 171883, NBRC 9655
Geosmithia viridis	IMI 288716, NBRC 33089
Glaciozyma antarctica	JCM 9057, NBRC 1917
Hamigera avellanea	IFM 52953, IMI 040230
Hamigera striata	IFM 52958, IMI 039741, JCM 23037
Hemicarpenteles paradoxus	IFM 48402, IMI 061446, JCM 2355, JCM 23003, NBRC 8172
Kurtzmanomyces insolitus	JCM 10409, NBRC 10839
Kurtzmanomyces tardus	JCM 10490
Lecanosticta acicola	IMI 281598
Leucosporidium creatinivorum	IFM 50467, JCM 10699
Leucosporidium fellii	JCM 9887
Leucosporidium fragarium	JCM 3930, NBRC 10411
Leucosporidium intermedium	IFM 49876, JCM 5291, NBRC 10178
Leucosporidium scottii	IFM 49208, JCM 9052, NBRC 1923
Leucosporidium yakuticum	JCM 10701
Monascus purpureus	IMI 210765, NBRC 4513
Neocarpenteles acanthosporum	IFM 42151, IMI 164621, NBRC 8939
Occultifur externus	JCM 10725
Paecilomyces aerugineus	IFM 49564, IMI 105412, JCM 23169
Penicillium abidjanum	IMI 136244
Penicillium adametzii	IMI 039751, JCM 9927
Penicillium alutaceum	IFO 31728, IMI 136243, NBRC 31728
Penicillium arenicola	IFM 49455, IMI 117658, JCM 9929
Penicillium atrovenetum	IFO 8138, IMI 061837
Penicillium camemberti	IFM 49450, IMI 027831, IMI 092200, JCM 22840
Penicillium canescens	IMI 28260, JCM 22758
Penicillium caperatum	IFM 42321, IMI 216895, NBRC 31730
Penicillium catenatum	IFM 49459, IMI 136241, NBRC 9577, NBRC 31774
Penicillium charlesii	IFM 49444, IMI 040232
Penicillium chrysogenum	IMI 024314, IMI 092208, JCM 22826, NBRC 32030
Penicillium cinnamopurpureum	IFM 48425, IMI 114483, JCM 22783, NBRC 31732
Penicillium citrinum	IFM 49447, IMI 091961
Penicillium corylophilum	IFO 7726, IMI 39817, NBRC 7726
Penicillium cryptum	IMI 296794
Penicillium dendriticum	IFM 49457, IMI 216897, NBRC 30686
Penicillium dimorphosporum	IMI 149680
Penicillium duclauxii	IFM 49456, IMI 040044
Penicillium erubescens	IFM 49460, IFO 31734, IMI 136204
Penicillium expansum	IFM 47463, IMI 039761
Penicillium fellutanum	IFM 49445, IFO 5761, IMI 039734
Penicillium fractum	IFM 42159, IMI 136701, NBRC 9023
Penicillium fuscum	IFO 7743, IMI 094209, JCM 22487
Penicillium glabrum	IMI 91944
Penicillium gracilentum	IMI 216900, NBRC 9699
Penicillium griseofulvum	IFM 47730, IMI 075832, NBRC 7641
Penicillium griseolum	IMI 071626, NBRC 8175
Penicillium herquei	IMI 28809, NBRC 31747
Penicillium idahoense	IMI 148393
Penicillium inusitatum	IFM 42166, IMI 136214, NBRC 9580
Penicillium isariiforme	IMI 060371
Penicillium islandicum	IMI 40042, NBRC 6963
Penicillium italicum	IFM 22856, IFM 49453, IMI 039760, NBRC 32032
Penicillium janthinellum	IMI 040238
Penicillium javanicum	IFM 42082, IMI 039733, NBRC 31735
Penicillium katangense	IFM 42154, IMI 136206, NBRC 9154
Penicillium kewense	IFM 42163, IMI 086561
Penicillium lanosum	IMI 040224, NBRC 5851
Penicillium lapidosum	IFM 49462, IMI 039743, NBRC 6100
Penicillium lassenii	IMI 148395, NBRC 9511, NBRC 31736
Penicillium marneffei	IMI 068794
Penicillium megasporum	IFM 49448, IMI 216904
Penicillium minioluteum	IFM 49458, IMI 089377
Penicillium namylowskii	IMI 40033, JCM 23031, NBRC 31149
Penicillium ochrosalmoneum	IMI 116248
Penicillium olsonii	IMI 192502, JCM 22857
Penicillium osmophilum	IMI 137977
Penicillium oxalicum	IFM 49446, IMI 192332
Penicillium ramusculum	IMI 063546
Penicillium restrictum	IFM 49441, IMI 040228
Penicillium sacculum	IMI 051498, NBRC 8114, NBRC 9454
Penicillium shearii	IMI 39739, NBRC 6088
Penicillium simplicissimum	IFM 42076, IMI 39816, NBRC 5762
Penicillium stipitatum	IMI 39805
Penicillium stolkiae	IMI 136210, NBRC 9157
Penicillium thomii	IFM 47735, IMI 068221, NBRC 6031
Penicillium tularense	IFM 42084, IMI 148394, NBRC 31740
Penicillium verrucosum	IMI 200310
Penicillium viridicatum	IFM 48057, IMI 039758, JCM 22829, NBRC 7736
Phialomyces macrosporus	IMI 110130
Phialosimplex chlamydosporus	IMI 387422
Phialosimplex sclerotialis	IAM 14794, JCM 23165
Platygloea disciformis	MAFF 235764, NBRC 32431
Polypaecilum insolitum	IFM 57489, IMI 075202, NBRC 8788
Rasamsonia argillacea	IMI 156096, JCM 23135, NBRC 31128
Rasamsonia cylindrospora	IMI 071623, JCM 23136, NBRC 8110
Rasamsonia emersonii	IFM 52961, IMI 116815, JCM 23024, NBRC 31232
Rhodosporidium azoricum	JCM 11251
Rhodosporidium babjevae	IFM 48570, IFM 48599, JCM 9279
Rhodosporidium diobovatum	IFM 48535, JCM 3787, NBRC 1830
Rhodosporidium fluviale	IFM 48601, JCM 10311
Rhodosporidium kratochvilovae	IFM 48536, JCM 8171
Rhodosporidium lusitaniae	IFM 48569, JCM 8547
Rhodosporidium paludigenum	IFM 48537, JCM 10292, NBRC 10547
Rhodosporidium sphaerocarpum	IFM 48538, JCM 8202, NBRC 1937
Rhodotorula araucariae	IFM 48501, JCM 3770, NBRC 10054
Rhodotorula armeniaca	IFM 48594, JCM 8977
Rhodotorula aurantiaca	IFM 40059, JCM 3771, NBRC 754
Rhodotorula auriculariae	IFM 48503, JCM 1597, NBRC 1580
Rhodotorula benthica	JCM 10901
Rhodotorula bogoriensis	IFM 48505, JCM 1692, NBRC 1966
Rhodotorula buffonii	IFM 48506, JCM 3929, NBRC 10268
Rhodotorula calyptogenae	JCM 10899
Rhodotorula cresolica	IFM 50680, JCM 10955
Rhodotorula diffluens	IFM 48507, JCM 1695, NBRC 10328
Rhodotorula ferulica	IFM 48509, JCM 8231, NBRC 10769
Rhodotorula foliorum	IFM 48510, JCM 1696, NBRC 10410
Rhodotorula glutinis	IFM 48391, IFM 57694, JCM 8208
Rhodotorula graminis	IFM 48392, JCM 3775, NBRC 10747
Rhodotorula hordea	JCM 3932, NBRC 10412
Rhodotorula hylophila	IFM 48513, JCM 1805, NBRC 10414
Rhodotorula ingeniosa	IFM 48514, JCM 9031, NBRC 10002
Rhodotorula javanica	IFM 48515, JCM 9032, NBRC 10285
Rhodotorula lysiniphila	JCM 5951
Rhodotorula marina	IFM 5761, JCM 3776, NBRC 928
Rhodotorula minuta	IFM 48518, JCM 3780, JCM 10953
Rhodotorula mucilaginosa	IFM 48530, JCM 8115
Rhodotorula nothofagi	IFM 48568, JCM 9034
Rhodotorula philyla	IFM 48521, JCM 3933, NBRC 10417
Rhodotorula phylloplana	IFM 48598, JCM 9035
Rhodotorula pilati	IFM 48522, JCM 9036, NBRC 10244
Rhodotorula pustula	IFM 48523, JCM 3934, NBRC 10248
Rhodotorula slooffiae	JCM 10954
Rhodotorula sonckii	IFM 48524, JCM 3935, NBRC 10420
Rhodotorula sp.	JCM 8162
Rhodotorula vanillica	IFM 50457, JCM 9741
Rhodotorula yarrowii	IFM 48682, IFM 49632, JCM 8232
Sagenomella bohemica	IAM 14789, JCM 23160
Sagenomella griseoviridis	IFM 51239, IMI 113160, JCM 23174
Sagenomella humicola	IFM 51241, IMI 113166, JCM 23164
Sagenomella striatispora	IFM 51338, IMI 113163, JCM 23166
Sagenomella verticillata	IAM 14697
Sakaguchia dacryoidea	IFM 48190, JCM 3795
Sclerocleista ornata	IFM 50948, IMI 055295, JCM 2354, JCM 23045
Sclerocleista thaxteri	IFM 42045, IFM 42047, IMI 055296, JCM 2264, NBRC 4042
Sporidiobolus pararoseus	JCM 5350
Sporidiobolus salmonicolor	JCM 1841
Sporidobolus microsporus	JCM 6882
Sporidobolus ruineniae	JCM 1839, NBRC 1689
Sporobolomyces alborubescens	JCM 5352, NBRC 10585
Sporobolomyces bischofiae	JCM 10338
Sporobolomyces blumeae	JCM 10212
Sporobolomyces carnicolor	IFM 48656, JCM 3766, NBRC 1107
Sporobolomyces coprosmae	JCM 8772
Sporobolomyces coprosmicola	JCM 8767
Sporobolomyces dimmenae	JCM 8762
Sporobolomyces diospyri	JCM 12157
Sporobolomyces falcatus	JCM 6838, NBRC 10587
Sporobolomyces fushanensis	JCM 12422
Sporobolomyces gracilis	JCM 2963, NBRC 1227
Sporobolomyces griseoflavus	JCM 5653, NBRC 10589
Sporobolomyces inositophilus	JCM 5654, NBRC 10590
Sporobolomyces kluyverinielii	JCM 6356, NBRC 10591
Sporobolomyces lactophilus	JCM 7595
Sporobolomyces linderae	JCM 8856
Sporobolomyces magnisporus	JCM 11898
Sporobolomyces marcillae	JCM 6883
Sporobolomyces novazealandicus	JCM 8756
Sporobolomyces nylandii	JCM 10213
Sporobolomyces odoratus	JCM 11641
Sporobolomyces ogasawarensis	JCM 10326
Sporobolomyces oryzicola	JCM 5299, NBRC 10180
Sporobolomyces phyllomatis	JCM 7549
Sporobolomyces poonsookiae	JCM 10207
Sporobolomyces pyrrosiae	JCM 12159
Sporobolomyces salicinus	JCM 2959, NBRC 10592
Sporobolomyces singularis	JCM 5356, NBRC 10594
Sporobolomyces subbrunneus	JCM 5278
Sporobolomyces syzygii	JCM 10337
Sporobolomyces taupoensis	JCM 8770
Sporobolomyces tsugae	JCM 2960, NBRC 10595
Sporobolomyces vermiculatus	JCM 10224
Sterigmatomyces elviae	IFM 48500, IFM 49894, JCM 1602, JCM 1822, NBRC 1843, NBRC 1912
Talaromyces bacillisporus	IFM 52959, IMI 040045
Talaromyces flavus	IFM 60672, IMI 197477
Talaromyces leycettanus	IMI 178525, JCM 12814
Talaromyces luteus	IFM 53168, IMI 089305, JCM 23134, NBRC 31753
Talaromyces purpureus	IFM 53865, IMI 181546, NBRC 31755
Talaromyces thermophilus	IFM 48410, IFM 52998, IMI 48593, NBRC 30541, NBRC 31798
Talaromyces trachyspermus	IFM 60673, IMI 040043, NBRC 31757
Talaromyces wortmanii	IFM 53866, IMI 040047
Thermoascus aurantiacus	IMI 173037, JCM 12816
Thermoascus crustaceus	IFM 60077, IMI 126333, JCM 12817
Thermoascus thermophilus	IFM 60075, IMI 123298
Thysanophora canadensis	IMI 137644
Trichoderma aeroaquaticum	NBRC 108031, NBRC 108034
Ustilago maydis	IFM 49220, JCM 2005
Warcupiella spinulosa	IMI 075885, JCM 2358, JCM 23046
Xeromyces bisporus	IMI 063718