Primer Detail
- Primer name
- ITS1
nu-SSU-1787-5' - Gene name
- ITS1-5.8S-ITS2 rRNA gene (ITS)
- Primer sequence (5'-3')
- TCCGTAGGTGAACCTGCGG
- Direction
- F
- Category
- universal primer
- Paired primer
- ITS4-Tul
- Reference
- White, T. J., Bruns, T., Lee, S. and Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications, eds. Innis, M. A., D. H. Gelfand, J. J. Sninsky, and T. J. White. Academic Press, Inc., New York. 315-322.
- Literature
- Baldoni DB, Coelho G, Jacques RJS, Silveira RMB, Grebenc T and Antoniolli ZI (2012) Brown rotting fungus closely related to Pseudomerulius curtisii (Boletales) recorded for the first time in South America. Mycosphere
- Rytas Vilgalys and Bao Lin Sun (1994) Ancient and recent patterns of geographic speciation in the oyster mushroom Pleurotus revealed by phylogenetic analysis of ribosomal DNA sequences. Proceedings of the National Academy of Sciences of the United States of America 91: 4599-4603.
- P.W. Crous, C.L. Schoch, K.D. Hyde, A.R. Wood, C. Gueidan, G.S. de Hoog and J.Z. Groenewald (2009) Phylogenetic lineages in the Capnodiales. Studies in Mycology 64: 17-47.
- M gardes and TD Bruns (1993) ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113-118.
- G. S. de Hoog and A. H. G. Gerrits van den Ende (1998) Molecular diagnostics of clinical strains of filamentous Basidiomycetes. Mycoses 41: 183-189.
- K. Tanaka, K. Hirayama, H. Yonezawa, S. Hatakeyama, Y. Harada, T. Sano, T. Shirouzu and T. Hosoya (2009) Molecular taxonomy of bambusicolous fungi: Tetraplosphaeriaceae, a new pleosporalean family with Tetraploa-like. Studies in Mycology 64: 175-209.
- Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61: 1323-1330.
- Mitchell, T.J., Freedman, E.Z., White, T.J., and Taylor, J.W. (1994) Unique oligonucleotide primers for the polymerase chain reaction to identify Cryptococcus neoformans. Journal of Clinical Microbiology 32: 253-255.
- A.J.L. Phillips, A. Alves, S.R. Pennycook, P.R. Johnston, A. Ramaley, A. Akulov and P.W. Crous (2008) Resolving the phylogenetic and taxonomic status of dark-spored teleomorph genera in the Botryosphaeriaceae. Persoonia 21: 29-55.
- Gargas, A. and DePriest, P. T. (1996) A nomenclature for fungal PCR primers with examples from intron-containing SSU rDNA. Mycologia 88: 745-748.
- Alves A, Crous PW, Correia A and Phillips AJL (2008) Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity 28: 1-13.
- http://www.lutzonilab.net/primers/page244.shtml
- Gao XX, Zhou H, Xu DY, Yu CH, Chen YQ and Qu LH (2005) High diversity of endophytic fungi from the pharmaceutical plant, Heterosmilax japonica Kunth revealed by cultivation independent approach. FEMS Microbiology Letters 249: 255-266.
- Valášková V and Baldrian P (2009) Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities. JournalPlant, Soil and Environment 55(10): 413-423.
- Arenz BE, Held BW, Jurgens JA, Farrell RL and Blanchette RA (2006) Fungal diversity in soils and historic wood from the Ross Sea Region of Antarctica. Soil Biology and Biochemistry 38: 3057-3064.
- Dirk Redecker (2000) Specific PCR primers to identify arbuscular mycorrhizal fungi within colonized roots. Mycorrhiza 10: 73-80.
- Bougoure DS and Cairney JWG (2005) Fungi associated with hair roots of Rhododendron lochiae (Ericaceae) in an Australian tropical cloud forest revealed by culturing and culture-independent molecular methods. Environmental Microbiology 7: 1743-1754.
- Mohamed Hijri, Dirk Redecker, Jean A. MacDonald-Comber Petetot, Kerstin Voigt, Johannes Wöstemeyer and Ian R. Sanders (2002) Identification and isolation of two Ascomycete fungi from spores of the arbuscular mycorrhizal fungus Scutellospora castanea. Applied and Environmental Microbiology 68(9): 4567-4573.
- Panelli S. Buffoni JN, Bonacina C and Feligini M (2012) Identification of moulds from the Taleggio cheese environment by the use of DNA barcodes. Food Control 28: 385-391.
- Figel IC, Marangoni PR, Tralamazza SM, Vicente VA, do Rocio, Dalzoto P, do Nascimento MM, de Hoog GS and Pimentel IC (2013) Black yeasts-like fungi isolated from dialysis water in hemodialysis units. Mycopathologia 175: 413-420.
- Crous PW, Groenewald JZ, Pongpanich K, Himaman W, Arzanlou M and Wingfield MJ (2004) Cryptic speciation and host specificity among Mycosphaerella spp. occurring on Australian Acacia species grown as exotics in the tropics. Studies in Mycology 50: 457-469.
- P Zalar, GS de Hoog, H-J Schroers, PW Crous, JZ Groenewald and N Gunde-Cimerman (2007) Phylogeny and ecology of the ubiquitous saprobe Cladosporium sphaerospermum, with descriptions of seven new species from hypersaline environments. Studies in Mycology 58: 157-183.
- Avila de la Calle A, Groenewald JZ, Trapero A and Crous PW (2005) Characterization and epitypification of Pseudocercospora cladosporioides, the causal organism of Cercospora leaf spot of olives. Mycological Research 109(8): 881-888.
- den Breeyen A, Groenewald JZ, Verkley GJM and Crous PW (2006) Morphological and molecular characterisation of Mycosphaerellaceae associated with the invasive weed, Chromolaena odorata. Fungal Diversity 23: 89-110.
- Abliz P, Fukushima K, Takizawa K, Nieda N, Miyaji M and Nishimura K (2003) Rapid identification of the genus fonsecaea by PCR with specific oligonucleotide primers. Journal of Clinical Microbiology 41(2): 873-876.
- Pedro W. Crous, Bernard Slippers, Michael J. Wingfield, John Rheeder, Walter FO Marasas, Alan JL Philips, Artur Alves, Treena Burgess, Paul Barber and Johannes Z Groenewald (2006) Phylogenetic lineages in the Botryosphaeriaceae. Studies in Mycology 55: 235-253.
- Irene Barnes, Pedro W. Crous, Brenda D. Wingfield and Michael J. Wingfield (2004) Multigene phylogenies reveal that red band needle blight of Pinus is caused by two distinct species of Dothistroma, D. septosporum and D. pini. Studies in Mycology 50: 551-565.
- Hambleton S, Tsuneda A and Currah RS (2003) Comparative morphology and phylogenetic placement of two microsclerotial black fungi from Sphagnum. Mycologia 95(5): 959-975.
- Pedro W Crous, Johannes Z Groenewald, Marizeth Groenewald, Pat Caldwell, Uwe Braun and Thomas C Harrington (2006) Species of Cercospora associated with grey leaf spot of maize. Studies in Mycology 55: 189-197.
- Groenewald JZ, Nakashima C, Nishikawa J, Shin HD, Park JH, Jama AN, Groenewald M, Braun U and Crous PW (2013) Species concepts in Cercospora: spotting the weeds among the roses. Studies in Mycology 75: 115-170.
- Groenewald M, Groenewald JZ and Crous PW (2005) Distinct species exist within the Cercospora apii morphotype. Phytopathology 95: 951-959.
- Feau N, Hamelin RC and Bernier L (2006) Attributes and congruence of three molecular data sets: Inferring phylogenies among Septoria-related species from woody perennial plants. Molecular Phylogenetics and Evolution 40: 808-829.
- Verkley GJ, Starink-Willemse M, van Iperen A and Abeln EC (2004) Phylogenetic analyses of Septoria species based on the ITS and LSU-D2 regions of nuclear ribosomal DNA. Mycologia 96: 558-571.
- ZM Kinyua, JJ Smith, GN Kibata, SA Simons and BC Langat (2010) Two Cercospora species associated with grey leaf spot disease in Kenya's maize production ecosystems. African Crop Science Journal 18: 183-194.
- M Arzanlou, JZ Groenewald, W Gams, U Braun, H-D Shin and PW Crous (2007) Phylogenetic and morphotaxonomic revision of Ramichloridium and allied genera. Studies in Mycology 58: 57-93.
- Grosch R, Schneider JH, Peth A, Waschke A, Franken P, Kofoet A and Jabaji-Hare SH (2007) Development of a specific PCR assay for the detection of Rhizoctonia solani AG 1-IB using SCAR primers. Journal of Applied Microbiology 102: 806-819.
- PA Okubara, KL Schroeder and TC Paulitz (2008) Identification and quantification of Rhizoctonia solani and R. oryzae using real-time polymerase chain reaction. Phytopathology 98: 837-847.
- Su'udi M, Park JM, Kang WR, Hwang DJ, Kim S and Ahn IP (2013) Quantification of rice sheath blight progression caused by Rhizoctonia solani. Journal of Microbiology 51: 380-388.
- Zhang J, Wu MD, Li GQ, Yang L, Yu L, Jiang DH, Huang HC and Zhuang WY (2010) Botrytis fabiopsis, a new species causing chocolate spot of broad bean in central China. Mycologia 102: 1114-1126.
- Amselem J, Cuomo CA, van Kan JA, Viaud M, Benito EP, Couloux A, Coutinho PM, de Vries RP, Dyer PS, Fillinger S, Fournier E, Gout L, Hahn M, Kohn L, Lapalu N, Plummer KM, Pradier JM, Quévillon E, Sharon A, Simon A, ten Have A, Tudzynski B, Tudzynski P, Wincker P, Andrew M, Anthouard V, Beever RE, Beffa R, Benoit I, Bouzid O, Brault B, Chen Z, Choquer M, Collémare J, Cotton P, Danchin EG, Da Silva C, Gautier A, Giraud C, Giraud T, Gonzalez C, Grossetete S, Güldener U, Henrissat B, Howlett BJ, Kodira C, Kretschmer M, Lappartient A, Leroch M, Levis C, Mauceli E, Neuvéglise C, Oeser B, Pearson M, Poulain J, Poussereau N, Quesneville H, Rascle C, Schumacher J, Ségurens B, Sexton A, Silva E, Sirven C, Soanes DM, Talbot NJ, Templeton M, Yandava C, Yarden O, Zeng Q, Rollins JA, Lebrun MH and Dickman M (2011) Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea. PLoS Genetics 7: e1002230.
- Holst-Jensen A, Kohn L, Jakobsen K and Schumacher T (1997) Molecular phylogeny and evolution of Monilinia (Sclerotiniaceae) based on coding and noncoding rDNA sequences. American Journal of Botany 84: 686-701.
- Amit Pandey, Partha Sarathi Mohanty and Pooja Arya (2010) Development of species specific primer for the early detection of Cylindrocladium quinqueseptatum causing leaf and seedling blight in Eucalyptus. Agriculture & Biology Journal of North America 1: 1253-1259.
- Rivera KG and Seifert KA (2011) A taxonomic and phylogenetic revision of the Penicillium sclerotiorum complex. Studies in Mycology 70: 139-158.
- Muthulekshmi Lajapathy Jeeva, Ajay Kumar Mishra, Pravi Vidyadharan, Raj Shekhar Misra and Vinayaka Hegde (2010) A species-specific polymerase chain reaction assay for rapid and sensitive detection of Sclerotium rolfsii. Australasian Plant Pathology 39: 517-523.
- Johanson A, Turner HC, McKay GJ and Brown AE (1998) A PCR-based method to distinguish fungi of the rice sheath-blight complex, Rhizoctonia solani, R. oryzae and R. oryzae-sativae. FEMS Microbiology Letters 15: 289-294.
- Daniel A Henk and Rytas Vilgalys (2007) Molecular phylogeny suggests a single origin of insect symbiosis in the Pucciniomycetes with support for some relationships within the genus Septobasidium. American Journal of Botany 94: 1515-1526.
- Mingzhu Li, Takahiro Asano, Haruhisa Suga and Koji Kageyama (2011) A Multiplex PCR for the Detection of Phytophthora nicotianae and P. cactorum, and a Survey of Their Occurrence in Strawberry Production Areas of Japan. Plant Disease 95: 1270-1278.
- Peter Bonants, Marjanne Hagenaar-de Weerdt, Marga van Gent-Pelzer, Isabelle Lacourt, David Cooke and Jim Duncan (1997) Detection and Identification of Phytophthora fragariae Hickman by the Polymerase Chain Reaction. European Journal of Plant Pathology 103: 345-355.
- Bhat RG and Browne GT (2010) Specific detection of Phytophthora cactorum in diseased strawberry plants using nested polymerase chain reaction. Plant Pathology 59: 121-129.
- Asano T, M Senda, H Suga and K Kageyama (2010) Development of multiplex PCR to detect five Pythium species related to turf-grass diseases. Journal of Phytopathology 158: 609-615.
- MPA Coetzee, BD Wingfield, T Kirisits, DB Chhetri, P Bloomer and MJ Wingfield (2005) Identification of Armillaria isolates from Bhutan based on DNA sequence comparisons. Plant Pathology 54: 36-45.
- A Shi, SK Kantartzi, MT Mmbaga, P Chen, F Mrema and E Nnodu (2008) PCR-based markers for detection of Colletotrichum acutatum and C. gloeosporioides in flowering dogwood (Cornus florida). Australasian Plant Pathology 37: 65-68.
- Toju H, Tanabe AS, Yamamoto S and Sato H (2012) High-coverage ITS primers for the DNA-based identification of ascomycetes and basidiomycetes in environmental samples. PLoS One 6: e40863.
- Klemsdal SS, Herrero ML, Wanner LA, Lund G and Hermansen A (2008) PCR-based identification of Pythium spp. causing cavity spot in carrots and sensitive detection in soil samples. Plant pathology 57: 877-886.
- Kawamura Y, Yokoo K, Tojo M and Hishiike M (2005) Distribution of Pythium porphyrae, the Causal Agent of Red Rot Disease of Porphyrae spp., in the Ariake Sea, Japan. Plant Disease 89: 1041-1047.
- K Kageyama, A Ohyama and M Hyakumachi (1997) Detection of Pythium ultimum using Ppolymerase chain reaction with species-specific primers. Plant Disease 81: 1155-1160.
- John J Weiland and Jamie L Sundsbak (2000) Differentiation and detection of sugar beet fungal pathogens using PCR amplification of actin coding sequences and the ITS region of the rRNA gene. Plant Disease 84: 475-482.
- Mingzhu Lia, Masako Sendab, Tsutomu Komatsuc, Haruhisa Sugad and Koji Kageyama (2010) Development of real-time PCR technique for the estimation of population density of Pythium intermedium in forest soils. Microbiological Research 165: 695-705.
- G Kernaghan, RD Reeleder and SMT Hoke (2008) Quantification of Pythium populations in ginseng soils by culture dependent and real-time PCR methods. Applied Soil Ecology 40: 447-455.
- K Tanaka, K Hirayama, H Yonezawa, G Sato, A Toriyabe, H Kudo, A Hashimoto, M Matsumura, Y Harada, Y Kurihara, T Shirouzu and T Hosoya (2015) Revision of the Massarineae (Pleosporales, Dothideomycetes). Studies in Mycology 82: 75-136.
- Reference Strain
Alternaria alternata IFM 41348 Aphanomyces iridis IFO 31934 Aquastroma magniostiolata JCM 19429, MAFF 243824 Aquilomyces rebunensis JCM 19427, MAFF 243862 Astrosphaeriella aggregata MAFF 239485, MAFF 239486 Astrosphaeriella stellata MAFF 239487 Aureobasidium pullulans IFM 4802 Bactrodesmium cubense JCM 14126 Candida albicans IFM 40214 Candida dubliniensis IFM 48313 Candida glabrata IFM 46888 Candida parapsilosis IFM 46863 Candida tropicalis IFM 46816 Capnobotryella renispora IAM 13014, IAM 13015, JCM 6932, JCM 6933, NBRC 32321, NBRC 32322 Cercospora apii IMI 077043, IMI 161116, MAFF 235978, MAFF 238072, MAFF 238299 Cercospora begoniae MAFF 237690 Cercospora beticola MAFF 238206, MAFF 305036 Cercospora capsici MAFF 238227 Cercospora citrullina MAFF 237872, MAFF 237913, MAFF 238205 Cercospora corchori MAFF 238191 Cercospora ipomoeae MAFF 239409 Cercospora kikuchii MAFF 305039, MAFF 305040 Cercospora lactucae-sativae MAFF 237719, MAFF 238209 Cercospora psophocarpicola MAFF 305757 Cercospora richardiicola MAFF 238210 Cercospora vignigena MAFF 237635 Cercospora zinniae MAFF 237718 Cladophialophora bantiana IFM 46165 Cladophialophora carrionii IFM 4805, IFM 4810, IFM 4812, IFM 41444, IFM 41446, IFM 41641 Cladophialophora devriesii IFM 51369 Cladophialophora minourae IFM 4841 Cladosporium cladosporioides IFM 41447, IFO 6368 Cladosporium colocasiae IFM 51371 Cladosporium coralloides IFM 41451, IFO 6536 Cladosporium elatum IFM 41452, IFO 6372 Cladosporium fulvum IFM 40703 Cladosporium herbarum IFM 41454 Cladosporium minusculum IFM 51370 Cladosporium resinae f. sp. avellaneum IFM 41456, IFO 6367 Cladosporium sphaerospermum IFM 41453, IFO 4458, IMI 49641, NBRC 6380 Cladosporium variabile IFM 41458, IFO 6378 Clypeoloculus akitaensis JCM 19424, MAFF 239467 Clypeoloculus hirosakiensis JCM 19425, MAFF 243864 Clypeoloculus microsporus JCM 19426, MAFF 243863 Clypeoloculus towadaensis JCM 19428, MAFF 243865 Cryptococcus neoformans var. neoformans IFM 40216 Dictyosporium aff. bulbosum JCM 19403, MAFF 243829 Dictyosporium aff. Heptasporum JCM 19406, MAFF 243828 Dictyosporium bulbosum MAFF 243835 Dictyosporium digitatum JCM 19404, JCM 19405, MAFF 243830, MAFF 243833, MAFF 243837 Dictyosporium hughesii JCM 19407, MAFF 243832 Dictyosporium pseudomusae JCM 19408, JCM 19409, MAFF 243831, MAFF 243836 Dictyosporium tetrasporum JCM 19410, MAFF 243834 Exophiala alcalophila IFM 41654 Exophiala dermatitidis IFM 41479, IFM 41818, IFM 41828, IFM 45986 Exophiala jeanselmei IFM 41691, IFM 45989 Exophiala moniliae IFM 41500 Exophiala spinifera IFM 4883 Fonsecaea compacta IFM 4886, IFM 41704, IFM 41931 Fonsecaea pedrosoi IFM 4856, IFM 4887, IFM 4889, IFM 4890, IFM 4892, IFM 4894, IFM 4896, IFM 4898, IFM 4914, IFM 4915 Fuscostagonospora sasae JCM 13104, MAFF 239614 Gregarithecium curvisporum JCM 19411, MAFF 243838 Helicascus aquaticus JCM 19423, MAFF 243866 Helicascus elaterascus MAFF 243867 Helicascus thalassioideus JCM 14147, JCM 17526, NBRC 107811 Helminthosporium dalbergiae MAFF 243853 Helminthosporium magnisporum MAFF 239278 Helminthosporium massarinum JCM 13094, JCM 13095, MAFF 239604, MAFF 239605 Helminthosporium sp. MAFF 243856, MAFF 243857, MAFF 243858 Helminthosporium velutinum MAFF 243854, MAFF 243855, MAFF 243859 Hormoconis resinae IFM 51372, IFO 8588 Hortaea werneckii IFM 51373 Kalmusia scabrispora JCM 12851, MAFF 239517, NBRC 106237 Katumotoa bambusicola JCM 13131, MAFF 239641 Keissleriella breviasca JCM 19413, JCM 19414, JCM 19415, MAFF 239476, MAFF 243843, MAFF 243844 Keissleriella culmifida JCM 19416, JCM 19417, MAFF 243848, MAFF 243849 Keissleriella gloeospora MAFF 239474 Keissleriella quadriseptata JCM 19418, MAFF 243850 Keissleriella sp. JCM 19420, MAFF 243845 Keissleriella taminensis MAFF 239475, MAFF 243846, MAFF 243847 Keissleriella yonaguniensis JCM 19419, MAFF 243851 Lasiodiplodia theobromae MAFF 238880 Lecythophora ho¡mannii IFM 4922 Lecythophora mutabilis IFM 4923 Lentithecium clioninum JCM 12703, MAFF 239293, MAFF 243839 Lentithecium pseudoclioninum JCM 19421, JCM 19422, MAFF 243840, MAFF 243841 Magnicamarosporium iriomotense JCM 19402, MAFF 243827 Malassezia furfur IFM 48585 Massarina arundinariae MAFF 239461, NBRC 106238, NBRC 106239 Massarina cisti JCM 14140 Massarina eburnea JCM 14422 Monodictys sp. MAFF 243825, MAFF 243826 Monodictys velatispora JCM 17529, JCM 17530, NBRC 107812, NBRC 107814 Myrmecridium schulzeri JCM 6967, JCM 6974, JCM 7234 Neokalmusia brevispora JCM 13543 Ophiosphaerella sasicola JCM 13134, MAFF 239644 Parabambusicola bambusina MAFF 239462, MAFF 243822, MAFF 243823 Paraphaeosphaeria michotii MAFF 243861 Periconia byssoides MAFF 243869, MAFF 243872, MAFF 243873 Periconia homothallica JCM 13100, MAFF 239610 Periconia igniaria JCM 14142 Periconia pseudobyssoides MAFF 243868, MAFF 243874 Periconia pseudodigitata MAFF 239674, MAFF 239675, MAFF 239676 Periconia sp. MAFF 243870, MAFF 243871 Phaeoacremonium parasiticum IFM 4924 Phaeosphaeria brevispora MAFF 239276, NBRC 106240 Phaeosphaeria sp. NBRC 106255 Phialophora alba IFM 51363, IFO 31973 Phialophora americana IFM 51361 Phialophora atrovirens IFM 51364, IFO 6793 Phialophora bubakii IFM 51365, IFO 6794 Phialophora cinerescens IFM 51366, IFO 6849 Phialophora fastigiata IFM 41577, IFO 6856 Phialophora heteromorpha IFM 41578, IFO 6878 Phialophora lagerbergii IFM 51367, IFO 8576 Phialophora melinii IFM 51362 Phialophora oxyspora IFM 51368 Phialophora repens IFM 4925 Phialophora richardsiae IFM 4926 Phialophora verrucosa IFM 4928, IFM 41710, IFM 41871, IFM 41873, IFM 41879 Phytophthora capsici IFO 30696, NBRC 30696 Phytophthora cinnamomi IFO 33183, NBRC 33182 Phytophthora cinnamoni MAFF 238147 Phytophthora megasperma IFO 31624 Phytophthora sojae NBRC 31016 Pleospora betae IMI 156653 Polyplosphaeria fusca JCM 13173, JCM 13175, JCM 13176, JCM 13177, MAFF 239683, MAFF 239685, MAFF 239686, MAFF 239687 Pseudocoleophoma calamagrostidis JCM 19412, MAFF 239468 Pseudotetraploa curviappendiculata JCM 12852, MAFF 239495, MAFF 239496, NBRC 106241 Pseudotetraploa javanica JCM 12854, MAFF 239498 Pseudotetraploa longissima JCM 12853, MAFF 239497 Pseudoxylomyces elegans MAFF 243852 Pythium acanthophoron MAFF 425319 Pythium dissotocum MAFF 305576 Pythium graminicola MAFF 305860, MAFF 425415 Pythium hydnosporum MAFF 305861, MAFF 305892 Pythium inflatum MAFF 305863, MAFF 425322 Pythium intermedium MAFF 305570, MAFF 425324 Pythium irregulare MAFF 305572, NBRC 100108 Pythium myriotylum NBRC 100113 Pythium nodosum MAFF 305905 Pythium paddicum NBRC 31993 Pythium pyrilobum NBRC 32560 Pythium spinosum NBRC 100116 Pythium sylvaticum NBRC 100119 Pythium torulosum MAFF 305575 Pythium ultimum NBRC 100123 Pythium volutum IFO 31926, IFO 31927 Quadricrura meridionalis NBRC 106242 Quadricrura septentrionalis NBRC 106243, NBRC 106244 Ramichloridium apiculatum JCM 6966, JCM 6972 Rhinocladiella aquaspersa IFM 4930 Rhinocladiella atrovirens IFM 4931 Rhinocladiella basitona IFM 47593 Roussoella hysterioides JCM 13126, MAFF 239636 Roussoella pustulans JCM 13127, MAFF 239637 Roussoella sp. NBRC 106245 Roussoellopsis sp. NBRC 106246 Roussoellopsis tosaensis JCM 13128, MAFF 239638 Saprolegnia parasitica NBRC 32708 Setoseptoria arundinacea MAFF 239460, MAFF 243842 Setoseptoria magniarundinacea JCM 13540, MAFF 239294 Spegazzinia deightonii MAFF 243876, MAFF 243877 Spegazzinia sp. MAFF 243878 Spegazzinia tessarthra MAFF 243875 Stagonospora berchemiae JCM 13101, MAFF 239611 Stagonospora perfecta JCM 13099, MAFF 239609 Stagonospora pseudoperfecta JCM 13097, MAFF 239607 Stagonospora sp. JCM 13098, MAFF 239608 Stagonospora tainanensis MAFF 243860 Tetraploa sp. JCM 14424, NBRC 106251 Tetraplosphaeria nagasakiensis JCM 13168, MAFF 239678 Tetraplosphaeria sasicola JCM 13167, MAFF 239677 Tingoldiago graminicola JCM 16485, JCM 16486, MAFF 239472, NBRC 106131, NBRC 106132 Trematosphaeria pertusa JCM 19430, MAFF 243879 Trichosporon asahii var. asahii IFM 48429 Triplosphaeria cylindrica JCM 13169, JCM 14425, MAFF 239679, NBRC 106247 Triplosphaeria maxima JCM 13172, MAFF 239682 Triplosphaeria sp. NBRC 106248, NBRC 106249 Trisplophaeria acuta JCM 13171, MAFF 239681 Versicolorisporium triseptatum JCM 14775