PriMicro Database

For classification, identification and molecular phylogenetic analyses of microbes

Primer Detail

Primer name
AsAPH2B
Gene name
ITS1-5.8S-ITS2 rRNA gene (ITS)
Primer sequence (5'-3')
GCGCGTTGTTCACAATAAATTGC
Direction
R
Category
specific primer
Paired primer
AsPyF
Reference
Asano T, M Senda, H Suga and K Kageyama (2010) Development of multiplex PCR to detect five Pythium species related to turf-grass diseases. Journal of Phytopathology 158: 609-615.
Literature
Schroeder KL, Martin F, de Cock AWAM, Lévesque CA, Spies C Okubara P and Paulitz TC (2013) Molecular detection and quantification of Pythium species - Evolving taxonomy, new tools and challenges. Plant Disease 97: 4-20.
Reference Strain
Aphanomyces iridisIFO 31934
Phytophthora capsiciNBRC 30696
Phytophthora megaspermaIFO 31624
Pythium acanthophoronMAFF 425319
Pythium dissotocumMAFF 305576
Pythium graminicolaMAFF 305860, MAFF 425415
Pythium hydnosporumMAFF 305861, MAFF 305892
Pythium inflatumMAFF 305863, MAFF 425322
Pythium intermediumMAFF 305570, MAFF 425324
Pythium irregulareMAFF 305572
Pythium nodosumMAFF 305905
Pythium torulosumMAFF 305575
Pythium volutumIFO 31926, IFO 31927
Saprolegnia parasiticaNBRC 32708