PriMicro Database

For classification, identification and molecular phylogenetic analyses of microbes

Primer Detail

Primer name
VANS1
Gene name
18S rRNA gene (SSU)
Primer sequence (5'-3')
GTCTAGTATAATCGTTATACAGG
Direction
F
Category
taxon specific
Target
vesicular-arbuscular endomycorrhizal fungus (level of order)
Paired primer
NSVA340
Reference
Simon L, Lalonde M and Bruns TD (1992) Specific amplification of 18S ribosomal genes from VA endomycorrhizal fungi colonizing roots. Applied and Environmental Microbiology 58: 291-295.
Literature
Dirk Redecker (2000) Specific PCR primers to identify arbuscular mycorrhizal fungi within colonized roots. Mycorrhiza 10: 73-80.
Clapp JP, Fitter AH and Young JPW (1999) Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp. Molecular Ecology 8: 915-921.
Dirk Redecker, Isabelle Hijri and Andres Wiemken (2003) Molecular identification of arbuscular mycorrhizal fungi in roots: Perspectives and problems. Folia Geobotanica 38: 113-124.
Clapp JP, Young JPW, Merryweather JW and Fitter AH (1995) Diversity of fungal symbionts in arbuscular mycorrhizas from a natural community. New Phytologist 130: 259-265.