PriMicro Database

For classification, identification and molecular phylogenetic analyses of microbes

Primer Detail

Primer name
EF4
Primer sequence (5'-3')
GGAAGGGRTGTATTTATTAG
Category
species specific
Target
fungi
Paired primer
VANS1 EF3 fung5 X158F
Reference
Eric Smit, Paula Leeflang, Boet Glandorf, Jan Dirk Van Elsas and Karel Wernars (1999) Analysis of fungal diversity in the wheat rhizosphere by sequencing of cloned PCR-amplified genes encoding 18S rRNA and temperature gradient gel electrophoresis. Applied and Environmental Microbiology 65(6): 2614-2621.
Literature
James Borneman and R. Jack Hartin (2000) PCR primers that amplify fungal rRNA genes from environmental samples. Applied and Environmental Microbiology 66(10): 4356-4360.
Valášková V and Baldrian P (2009) Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities. JournalPlant, Soil and Environment 55(10): 413-423.