PriMicro Database

For classification, identification and molecular phylogenetic analyses of microbes

Primer Detail

Primer name
fRPB2-5F
FRPB2-5f
f5F
Gene name
RNA polymerase II second largest subunit (RPB2)
Primer sequence (5'-3')
GAYGAYMGWGATCAYTTYGG
Direction
F
Category
fungus-specific
Target
fungi
Reference
Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16: 1799-1808.
Literature
Valérie Reeb, François Lutzoni and Claude Roux (2004) Contribution of RPB2 to multilocus phylogenetic studies of the euascomycetes (Pezizomycotina, Fungi) with special emphasis on the lichen-forming Acarosporaceae and evolution of polyspory. Molecular Phylogenetics and Evolution 32: 1036-1060.
EWA Boehm, GK Mugambi, AN Miller, SM Huhndorf, S Marincowitz, JW Spatafora and CL Schoch (2009) A molecular phylogenetic reappraisal of the Hysteriaceae, Mytilinidiaceae and Gloniaceae (Pleosporomycetidae, Dothideomycetes) with keys to world species. Studies in Mycology 64: 49-83.
Tazick Z and Tajick Ghanbary MA (2013) Taxonomic position of Bipolaris oryzae among other Cochliobolus species using Ribosomal region and some Protein Coding genes. Research Journal of Recent Sciences 2: 212-216.
Conrad L. Schocha, Keith A. Seifertb, Sabine Huhndorfc, Vincent Robertd, John L. Spougea, C. André Levesqueb, Wen Chenb and Fungal Barcoding Consortium (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences
Houbraken J and Samson RA (2011) Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Studies in Mycology 70: 1-51.
M Réblová, W Gams and KA Seifert (2011) Monilochaetes and allied genera of the Glomerellales, and a reconsideration of families in the Microascales. Studies in Mycology 68: 163-191.
P. Brandon Matheny (2005) Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe; Agaricales). Molecular Phylogenetics and Evolution 35: 1-20.
Verkley GJ, Quaedvlieg W, Shin HD and Crous PW (2013) A new approach to species delimitation in Septoria. Studies in Mycology 75: 213-305.
Quaedvlieg W, Verkley GJ, Shin HD, Barreto RW, Alfenas AC, Swart WJ, Groenewald JZ and Crous PW (2013) Sizing up Septoria. Studies in Mycology 75: 307-390.
Quaedvlieg W, Groenewald JZ, Jesús Yáñez-Morales M and Crous PW (2012) DNA barcoding of Mycosphaerella species of quarantine importance to Europe. Persoonia 29: 101-115.
Stukenbrock EH, Quaedvlieg W, Javan-Nikhah M, Zala M, Crous PW, (2012) Zymoseptoria ardabilia and Z. pseudotritici, two progenitor species of the septoria tritici leaf blotch fungus Z. tritici (synonym: Mycosphaerella graminicola). Mycologia 104: 1397-1407.
Quaedvlieg W, Kema GHJ, Groenewald JZ, Verkley GJM, Seifbarghi S, Razavi M, Gohari A, Mirzadi, Mehrabi R and Crous PW (2011) Zymoseptoria gen. nov.: a new genus to accommodate Septoria-like species occurring on graminicolous hosts. Persoonia 26: 57-69.
Bischoff JF, Rehner SA and Humber RA (2006) Metarhizium frigidum sp. nov.: a cryptic species of M. anisopliae and a member of the M. flavoviride complex. Mycologia 98: 737-745.
Matheny PB, Wang Z, Binder M, Curtis JM, Lim YW, Nilsson RH, Hughes KW, Hofstetter V, Ammirati JF, Schoch CL, Langer E, Langer G, McLaughlin DJ, Wilson AW, Frøslev T, Ge ZW, Kerrigan RW, Slot JC, Yang ZL, Baroni TJ, Fischer M, Hosaka K, Matsuura K, Seidl MT, Vauras J and Hibbett DS (2007) Contributions of rpb2 and tef1 to the phylogeny of mushrooms and allies (Basidiomycota, Fungi). Molecular Phylogenetics and Evolution 43: 430-451.
X-Z Liu, Q-M Wang, B Theelen, M Groenewald, F-Y Bai and T Boekhout (2015) Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses. Studies in Mycology 81: 1-26.
Q-M Wang, M Groenewald, M Takashima, B Theelen, P-J Han, X-Z Liu, T Boekhout and F-Y Bai (2015) Phylogeny of yeasts and related filamentous fungi within Pucciniomycotina determined from multigene sequence analyses. Studies in Mycology 81: 27-53.
Q-M Wang, B Theelen, M Groenewald, F-Y Bai and T Boekhout (2014) Moniliellomycetes and Malasseziomycetes, two new classes in Ustilaginomycotina. Persoonia 33: 41-47.
WM Jaklitsch and H Voglmayr (2015) Biodiversity of Trichoderma (Hypocreaceae) in Southern Europe and Macaronesia. Studies in Mycology 80: 1-87.
Walter M Jaklitsch, Monika Komon, Christian P Kubicek and Irina S Druzhinina (2005) Hypocrea voglmayrii sp. nov. from the Austrian Alps represents a new phylogenetic clade in Hypocrea/Trichoderma. Mycologia 97: 1365-1378.
Priscila Chaverri and Gary J Samuels (2003) Hypocrea/Trichoderma (Ascomycota, Hypocreales, Hypocreaceae): species with green ascospores. Studies in Mycology 48: 1-116.
Reference Strain
Aspergillus aculeatusIFM 47778, IMI 211388, JCM 22898
Aspergillus amylovorusIMI 129961
Aspergillus arenariusIMI 055632
Aspergillus avenaceusIFM 49793, IMI 016140, JCM 2719, NBRC 7539, NBRC 8129
Aspergillus biplanusIMI 235602
Aspergillus brunneouniseriatusIMI 227677
Aspergillus candidusIMI 091889
Aspergillus cervinusIMI 107684, JCM 1722
Aspergillus clavatoflavusIFM 49795, IMI 124937
Aspergillus clavatusIMI 15949
Aspergillus conjunctusIMI 135421
Aspergillus egyptiacusIMI 141415
Aspergillus funiculosusIFM 50946, IMI 054397, JCM 2352, NBRC 8131
Aspergillus janusIMI 016065
Aspergillus kanagawaensisIMI 126690, JCM 1723, NBRC 6219
Aspergillus ochraceusIMI 016247, JCM 1958
Aspergillus penicilliformisIMI 129968
Aspergillus pulvinusIMI 139628
Aspergillus restrictusIMI 016267, JCM 1727
Aspergillus sparsusIMI 019394, JCM 2357
Aspergillus versicolorIMI 45554, NBRC 30338
Aspergillus wentiiIMI 017295
Aspergillus zonatusIFM 50940, IMI 124936, JCM 2254, NBRC 8817
Bannoa hahajimensisJCM 10336
Basipetospora halophilicaIFO 9650
Bensingtonia ingoldiiJCM 7445, NBRC 10578
Bensingtonia miscanthiJCM 5733, NBRC 10579
Bensingtonia musaeIFM 49877, JCM 8801, NBRC 102559
Bensingtonia naganoensisJCM 5978, NBRC 10580
Bensingtonia phylladaJCM 7476, NBRC 10581
Bensingtonia sakaguchiiJCM 10047, NBRC 102558
Bensingtonia subroseaIFM 57755, JCM 5735, NBRC 10582
Bensingtonia thailandicaJCM 10651, NBRC 102560
Bensingtonia yuccicolaIFM 49223, JCM 6251, NBRC 10596
Chrysosporium inopsIMI 096729, NBRC 7583
Curvibasidium cygneicollumIFM 48566, IFM 48595, JCM 9029, JCM 10310, NBRC 100752
Eupenicillium hirayamaeIFM 48426, IMI 78255, JCM 22785, NBRC 6435
Eurotium amstelodamiIFM 47794, IMI 229971, JCM 1565, JCM 10260, NBRC 33018
Eurotium herbariorumIMI 211383, JCM 1575
Fennellia flavipesIFM 40894, IMI 171883, NBRC 9655
Geosmithia viridisIMI 288716, NBRC 33089
Glaciozyma antarcticaJCM 9057, NBRC 1917
Hamigera avellaneaIFM 52953, IMI 040230
Hamigera striataIFM 52958, IMI 039741, JCM 23037
Hemicarpenteles paradoxusIFM 48402, IMI 061446, JCM 2355, JCM 23003, NBRC 8172
Kurtzmanomyces insolitusJCM 10409, NBRC 10839
Kurtzmanomyces tardusJCM 10490
Lecanosticta acicolaIMI 281598
Leucosporidium creatinivorumIFM 50467, JCM 10699
Leucosporidium felliiJCM 9887
Leucosporidium fragariumJCM 3930, NBRC 10411
Leucosporidium intermediumIFM 49876, JCM 5291, NBRC 10178
Leucosporidium scottiiIFM 49208, JCM 9052, NBRC 1923
Leucosporidium yakuticumJCM 10701
Monascus purpureusIMI 210765, NBRC 4513
Neocarpenteles acanthosporumIFM 42151, IMI 164621, NBRC 8939
Occultifur externusJCM 10725
Paecilomyces aerugineusIFM 49564, IMI 105412, JCM 23169
Penicillium abidjanumIMI 136244
Penicillium adametziiIMI 039751, JCM 9927
Penicillium alutaceumIFO 31728, IMI 136243, NBRC 31728
Penicillium arenicolaIFM 49455, IMI 117658, JCM 9929
Penicillium atrovenetumIFO 8138, IMI 061837
Penicillium camembertiIFM 49450, IMI 027831, IMI 092200, JCM 22840
Penicillium canescensIMI 28260, JCM 22758
Penicillium caperatumIFM 42321, IMI 216895, NBRC 31730
Penicillium catenatumIFM 49459, IMI 136241, NBRC 9577, NBRC 31774
Penicillium charlesiiIFM 49444, IMI 040232
Penicillium chrysogenumIMI 024314, IMI 092208, JCM 22826, NBRC 32030
Penicillium cinnamopurpureumIFM 48425, IMI 114483, JCM 22783, NBRC 31732
Penicillium citrinumIFM 49447, IMI 091961
Penicillium corylophilumIFO 7726, IMI 39817, NBRC 7726
Penicillium cryptumIMI 296794
Penicillium dendriticumIFM 49457, IMI 216897, NBRC 30686
Penicillium dimorphosporumIMI 149680
Penicillium duclauxiiIFM 49456, IMI 040044
Penicillium erubescensIFM 49460, IFO 31734, IMI 136204
Penicillium expansumIFM 47463, IMI 039761
Penicillium fellutanumIFM 49445, IFO 5761, IMI 039734
Penicillium fractumIFM 42159, IMI 136701, NBRC 9023
Penicillium fuscumIFO 7743, IMI 094209, JCM 22487
Penicillium glabrumIMI 91944
Penicillium gracilentumIMI 216900, NBRC 9699
Penicillium griseofulvumIFM 47730, IMI 075832, NBRC 7641
Penicillium griseolumIMI 071626, NBRC 8175
Penicillium herqueiIMI 28809, NBRC 31747
Penicillium idahoenseIMI 148393
Penicillium inusitatumIFM 42166, IMI 136214, NBRC 9580
Penicillium isariiformeIMI 060371
Penicillium islandicumIMI 40042, NBRC 6963
Penicillium italicumIFM 22856, IFM 49453, IMI 039760, NBRC 32032
Penicillium janthinellumIMI 040238
Penicillium javanicumIFM 42082, IMI 039733, NBRC 31735
Penicillium katangenseIFM 42154, IMI 136206, NBRC 9154
Penicillium kewenseIFM 42163, IMI 086561
Penicillium lanosumIMI 040224, NBRC 5851
Penicillium lapidosumIFM 49462, IMI 039743, NBRC 6100
Penicillium lasseniiIMI 148395, NBRC 9511, NBRC 31736
Penicillium marneffeiIMI 068794
Penicillium megasporumIFM 49448, IMI 216904
Penicillium minioluteumIFM 49458, IMI 089377
Penicillium namylowskiiIMI 40033, JCM 23031, NBRC 31149
Penicillium ochrosalmoneumIMI 116248
Penicillium olsoniiIMI 192502, JCM 22857
Penicillium osmophilumIMI 137977
Penicillium oxalicumIFM 49446, IMI 192332
Penicillium ramusculumIMI 063546
Penicillium restrictumIFM 49441, IMI 040228
Penicillium sacculumIMI 051498, NBRC 8114, NBRC 9454
Penicillium sheariiIMI 39739, NBRC 6088
Penicillium simplicissimumIFM 42076, IMI 39816, NBRC 5762
Penicillium stipitatumIMI 39805
Penicillium stolkiaeIMI 136210, NBRC 9157
Penicillium thomiiIFM 47735, IMI 068221, NBRC 6031
Penicillium tularenseIFM 42084, IMI 148394, NBRC 31740
Penicillium verrucosumIMI 200310
Penicillium viridicatumIFM 48057, IMI 039758, JCM 22829, NBRC 7736
Phialomyces macrosporusIMI 110130
Phialosimplex chlamydosporusIMI 387422
Phialosimplex sclerotialisIAM 14794, JCM 23165
Platygloea disciformisMAFF 235764, NBRC 32431
Polypaecilum insolitumIFM 57489, IMI 075202, NBRC 8788
Rasamsonia argillaceaIMI 156096, JCM 23135, NBRC 31128
Rasamsonia cylindrosporaIMI 071623, JCM 23136, NBRC 8110
Rasamsonia emersoniiIFM 52961, IMI 116815, JCM 23024, NBRC 31232
Rhodosporidium azoricumJCM 11251
Rhodosporidium babjevaeIFM 48570, IFM 48599, JCM 9279
Rhodosporidium diobovatumIFM 48535, JCM 3787, NBRC 1830
Rhodosporidium fluvialeIFM 48601, JCM 10311
Rhodosporidium kratochvilovaeIFM 48536, JCM 8171
Rhodosporidium lusitaniaeIFM 48569, JCM 8547
Rhodosporidium paludigenumIFM 48537, JCM 10292, NBRC 10547
Rhodosporidium sphaerocarpumIFM 48538, JCM 8202, NBRC 1937
Rhodotorula araucariaeIFM 48501, JCM 3770, NBRC 10054
Rhodotorula armeniacaIFM 48594, JCM 8977
Rhodotorula aurantiacaIFM 40059, JCM 3771, NBRC 754
Rhodotorula auriculariaeIFM 48503, JCM 1597, NBRC 1580
Rhodotorula benthicaJCM 10901
Rhodotorula bogoriensisIFM 48505, JCM 1692, NBRC 1966
Rhodotorula buffoniiIFM 48506, JCM 3929, NBRC 10268
Rhodotorula calyptogenaeJCM 10899
Rhodotorula cresolicaIFM 50680, JCM 10955
Rhodotorula diffluensIFM 48507, JCM 1695, NBRC 10328
Rhodotorula ferulicaIFM 48509, JCM 8231, NBRC 10769
Rhodotorula foliorumIFM 48510, JCM 1696, NBRC 10410
Rhodotorula glutinisIFM 48391, IFM 57694, JCM 8208
Rhodotorula graminisIFM 48392, JCM 3775, NBRC 10747
Rhodotorula hordeaJCM 3932, NBRC 10412
Rhodotorula hylophilaIFM 48513, JCM 1805, NBRC 10414
Rhodotorula ingeniosaIFM 48514, JCM 9031, NBRC 10002
Rhodotorula javanicaIFM 48515, JCM 9032, NBRC 10285
Rhodotorula lysiniphilaJCM 5951
Rhodotorula marinaIFM 5761, JCM 3776, NBRC 928
Rhodotorula minutaIFM 48518, JCM 3780, JCM 10953
Rhodotorula mucilaginosaIFM 48530, JCM 8115
Rhodotorula nothofagiIFM 48568, JCM 9034
Rhodotorula philylaIFM 48521, JCM 3933, NBRC 10417
Rhodotorula phylloplanaIFM 48598, JCM 9035
Rhodotorula pilatiIFM 48522, JCM 9036, NBRC 10244
Rhodotorula pustulaIFM 48523, JCM 3934, NBRC 10248
Rhodotorula slooffiaeJCM 10954
Rhodotorula sonckiiIFM 48524, JCM 3935, NBRC 10420
Rhodotorula sp.JCM 8162
Rhodotorula vanillicaIFM 50457, JCM 9741
Rhodotorula yarrowiiIFM 48682, IFM 49632, JCM 8232
Sagenomella bohemicaIAM 14789, JCM 23160
Sagenomella griseoviridisIFM 51239, IMI 113160, JCM 23174
Sagenomella humicolaIFM 51241, IMI 113166, JCM 23164
Sagenomella striatisporaIFM 51338, IMI 113163, JCM 23166
Sagenomella verticillataIAM 14697
Sakaguchia dacryoideaIFM 48190, JCM 3795
Sclerocleista ornataIFM 50948, IMI 055295, JCM 2354, JCM 23045
Sclerocleista thaxteriIFM 42045, IFM 42047, IMI 055296, JCM 2264, NBRC 4042
Sporidiobolus pararoseusJCM 5350
Sporidiobolus salmonicolorJCM 1841
Sporidobolus microsporusJCM 6882
Sporidobolus ruineniaeJCM 1839, NBRC 1689
Sporobolomyces alborubescensJCM 5352, NBRC 10585
Sporobolomyces bischofiaeJCM 10338
Sporobolomyces blumeaeJCM 10212
Sporobolomyces carnicolorIFM 48656, JCM 3766, NBRC 1107
Sporobolomyces coprosmaeJCM 8772
Sporobolomyces coprosmicolaJCM 8767
Sporobolomyces dimmenaeJCM 8762
Sporobolomyces diospyriJCM 12157
Sporobolomyces falcatusJCM 6838, NBRC 10587
Sporobolomyces fushanensisJCM 12422
Sporobolomyces gracilisJCM 2963, NBRC 1227
Sporobolomyces griseoflavusJCM 5653, NBRC 10589
Sporobolomyces inositophilusJCM 5654, NBRC 10590
Sporobolomyces kluyverinieliiJCM 6356, NBRC 10591
Sporobolomyces lactophilusJCM 7595
Sporobolomyces linderaeJCM 8856
Sporobolomyces magnisporusJCM 11898
Sporobolomyces marcillaeJCM 6883
Sporobolomyces novazealandicusJCM 8756
Sporobolomyces nylandiiJCM 10213
Sporobolomyces odoratusJCM 11641
Sporobolomyces ogasawarensisJCM 10326
Sporobolomyces oryzicolaJCM 5299, NBRC 10180
Sporobolomyces phyllomatisJCM 7549
Sporobolomyces poonsookiaeJCM 10207
Sporobolomyces pyrrosiaeJCM 12159
Sporobolomyces salicinusJCM 2959, NBRC 10592
Sporobolomyces singularisJCM 5356, NBRC 10594
Sporobolomyces subbrunneusJCM 5278
Sporobolomyces syzygiiJCM 10337
Sporobolomyces taupoensisJCM 8770
Sporobolomyces tsugaeJCM 2960, NBRC 10595
Sporobolomyces vermiculatusJCM 10224
Sterigmatomyces elviaeIFM 48500, IFM 49894, JCM 1602, JCM 1822, NBRC 1843, NBRC 1912
Talaromyces bacillisporusIFM 52959, IMI 040045
Talaromyces flavusIFM 60672, IMI 197477
Talaromyces leycettanusIMI 178525, JCM 12814
Talaromyces luteusIFM 53168, IMI 089305, JCM 23134, NBRC 31753
Talaromyces purpureusIFM 53865, IMI 181546, NBRC 31755
Talaromyces thermophilusIFM 48410, IFM 52998, IMI 48593, NBRC 30541, NBRC 31798
Talaromyces trachyspermusIFM 60673, IMI 040043, NBRC 31757
Talaromyces wortmaniiIFM 53866, IMI 040047
Thermoascus aurantiacusIMI 173037, JCM 12816
Thermoascus crustaceusIFM 60077, IMI 126333, JCM 12817
Thermoascus thermophilusIFM 60075, IMI 123298
Thysanophora canadensisIMI 137644
Trichoderma aeroaquaticumNBRC 108031, NBRC 108034
Ustilago maydisIFM 49220, JCM 2005
Warcupiella spinulosaIMI 075885, JCM 2358, JCM 23046
Xeromyces bisporusIMI 063718