PriMicro Database

For classification, identification and molecular phylogenetic analyses of microbes

Primer Detail

Primer name
Gene name
actin gene (ACT)
Primer sequence (5'-3')
filamentous ascomycetes
Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553-556.
K Bensch, U Braun, JZ Groenewald and PW Crous (2012) The genus Cladosporium. Studies in Mycology 72: 1-401.
Crous PW, Groenewald JZ, Pongpanich K, Himaman W, Arzanlou M and Wingfield MJ (2004) Cryptic speciation and host specificity among Mycosphaerella spp. occurring on Australian Acacia species grown as exotics in the tropics. Studies in Mycology 50: 457-469.
P Zalar, GS de Hoog, H-J Schroers, PW Crous, JZ Groenewald and N Gunde-Cimerman (2007) Phylogeny and ecology of the ubiquitous saprobe Cladosporium sphaerospermum, with descriptions of seven new species from hypersaline environments. Studies in Mycology 58: 157-183.
Avila de la Calle A, Groenewald JZ, Trapero A and Crous PW (2005) Characterization and epitypification of Pseudocercospora cladosporioides, the causal organism of Cercospora leaf spot of olives. Mycological Research 109(8): 881-888.
Crous PW, Tanaka K, Summerell BA and Groenewald JZ (2011) Additions to the Mycosphaerella complex. IMA Fungus 2(1): 49-64.
Pedro W Crous, Johannes Z Groenewald, Marizeth Groenewald, Pat Caldwell, Uwe Braun and Thomas C Harrington (2006) Species of Cercospora associated with grey leaf spot of maize. Studies in Mycology 55: 189-197.
Groenewald JZ, Nakashima C, Nishikawa J, Shin HD, Park JH, Jama AN, Groenewald M, Braun U and Crous PW (2013) Species concepts in Cercospora: spotting the weeds among the roses. Studies in Mycology 75: 115-170.
Groenewald M, Groenewald JZ and Crous PW (2005) Distinct species exist within the Cercospora apii morphotype. Phytopathology 95: 951-959.
L Lombard, PW Crous, BD Wingfield and MJ Wingfield (2010) Phylogeny and systematics of the genus Calonectria. Studies in Mycology 66: 31-69.
Aveskamp MM, Verkley GJM, Gruyter J de, Murace MA, Perello A, Woudenberg JHC, Groenewald JZ and Crous PW (2009) DNA phylogeny reveals polyphyly of Phoma section Peyronellaea and multiple taxonomic novelties. Mycologia 101: 363-382.
Amselem J, Cuomo CA, van Kan JA, Viaud M, Benito EP, Couloux A, Coutinho PM, de Vries RP, Dyer PS, Fillinger S, Fournier E, Gout L, Hahn M, Kohn L, Lapalu N, Plummer KM, Pradier JM, Quévillon E, Sharon A, Simon A, ten Have A, Tudzynski B, Tudzynski P, Wincker P, Andrew M, Anthouard V, Beever RE, Beffa R, Benoit I, Bouzid O, Brault B, Chen Z, Choquer M, Collémare J, Cotton P, Danchin EG, Da Silva C, Gautier A, Giraud C, Giraud T, Gonzalez C, Grossetete S, Güldener U, Henrissat B, Howlett BJ, Kodira C, Kretschmer M, Lappartient A, Leroch M, Levis C, Mauceli E, Neuvéglise C, Oeser B, Pearson M, Poulain J, Poussereau N, Quesneville H, Rascle C, Schumacher J, Ségurens B, Sexton A, Silva E, Sirven C, Soanes DM, Talbot NJ, Templeton M, Yandava C, Yarden O, Zeng Q, Rollins JA, Lebrun MH and Dickman M (2011) Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea. PLoS Genetics 7: e1002230.
BS Weir, PR Johnston and U Damm (2012) The Colletotrichum gloeosporioides species complex. Studies in Mycology 73: 115-180.
Collado-Romero M, Mercado-Blanco J, Olivares-García C and Jiménez-Díaz RM (2008) Phylogenetic analysis of Verticillium dahliae vegetative compatibility groups. Phytopathology 98: 1019-1028.
U Damm, PF Cannon, JHC Woudenberg and PW Crous (2012) The Colletotrichum acutatum species complex. Studies in Mycology 73: 37-113.
Toyozo Sato and Jouji Moriwaki (2013) Molecular re-identification of strains in NIAS Genebank belonging to phylogenetic groups A2 and A4 of the Colletotrichum acutatum species complex. Microbiology and Culture Collections 29: 13-23.
Reference Strain
Cercospora apiiIMI 077043, IMI 161116, MAFF 235978, MAFF 238072, MAFF 238299
Cercospora begoniaeMAFF 237690
Cercospora beticolaMAFF 238206, MAFF 305036
Cercospora capsiciMAFF 238227
Cercospora citrullinaMAFF 237872, MAFF 237913, MAFF 238205
Cercospora corchoriMAFF 238191
Cercospora ipomoeaeMAFF 239409
Cercospora kikuchiiMAFF 305039, MAFF 305040
Cercospora lactucae-sativaeMAFF 237719, MAFF 238209
Cercospora psophocarpicolaMAFF 305757
Cercospora richardiicolaMAFF 238210
Cercospora vignigenaMAFF 237635
Cercospora zinniaeMAFF 237718
Cladosporium sphaerospermumIMI 49641, NBRC 6380
Colletotrichum acutatumMAFF 238555, MAFF 744062, MAFF 744063
Colletotrichum carthamiMAFF 239356, MAFF 239357, MAFF 239358, MAFF 239359, MAFF 239360, MAFF 239361, MAFF 239362, MAFF 239364, MAFF 239365, MAFF 239366, MAFF 239367, MAFF 239368, MAFF 239369, MAFF 239370, MAFF 239371, MAFF 239372, MAFF 239373, MAFF 239374
Colletotrichum godetiaeMAFF 240289, MAFF 241295, MAFF 241296, MAFF 241297, MAFF 306506
Colletotrichum nymphaeaeMAFF 239773, MAFF 241261, MAFF 241294, MAFF 242413, MAFF 242414, MAFF 242415, MAFF 242416, MAFF 242417, MAFF 242418, MAFF 242419, MAFF 242581, MAFF 242590, MAFF 306406, MAFF 306407, MAFF 306430, MAFF 306487, MAFF 306488, MAFF 306503, MAFF 306505
Colletotrichum scovilleiMAFF 242420, MAFF 242421, MAFF 242422, MAFF 242425, MAFF 242426, MAFF 242427, MAFF 242428, MAFF 242592, MAFF 242692, MAFF 242693, MAFF 243021, MAFF 243022, MAFF 243038
Colletotrichum sloaneiMAFF 239736
Colletotrichum sp.(B)MAFF 237894, MAFF 306172
Colletotrichum sp.(C)MAFF 240237
Colletotrichum sp.(J)MAFF 237922, MAFF 306725, MAFF 306726, MAFF 410809
Lasiodiplodia theobromaeMAFF 238880
Sphaerulina myriadeaJCM 15565