PriMicro Database

For classification, identification and molecular phylogenetic analyses of microbes

Primer Detail

Primer name
Gene name
translation elongation factor 1 alpha (EF-1α/TEF)
Primer sequence (5'-3')
filamentous ascomycetes
Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553-556.
K. Tanaka, K. Hirayama, H. Yonezawa, S. Hatakeyama, Y. Harada, T. Sano, T. Shirouzu and T. Hosoya (2009) Molecular taxonomy of bambusicolous fungi: Tetraplosphaeriaceae, a new pleosporalean family with Tetraploa-like. Studies in Mycology 64: 175-209.
Alves A, Crous PW, Correia A and Phillips AJL (2008) Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity 28: 1-13.
K Bensch, U Braun, JZ Groenewald and PW Crous (2012) The genus Cladosporium. Studies in Mycology 72: 1-401.
Crous PW, Groenewald JZ, Pongpanich K, Himaman W, Arzanlou M and Wingfield MJ (2004) Cryptic speciation and host specificity among Mycosphaerella spp. occurring on Australian Acacia species grown as exotics in the tropics. Studies in Mycology 50: 457-469.
Crous PW, Groenewald JZ, Risède J-M, Simoneau P and Hywel-Jones NL (2004) Calonectria species and their Cylindrocladium anamorphs: species with sphaeropedunculate vesicles. Studies in Mycology 50: 415-430.
Crous PW, Tanaka K, Summerell BA and Groenewald JZ (2011) Additions to the Mycosphaerella complex. IMA Fungus 2(1): 49-64.
Irene Barnes, Pedro W. Crous, Brenda D. Wingfield and Michael J. Wingfield (2004) Multigene phylogenies reveal that red band needle blight of Pinus is caused by two distinct species of Dothistroma, D. septosporum and D. pini. Studies in Mycology 50: 551-565.
Pedro W Crous, Johannes Z Groenewald, Marizeth Groenewald, Pat Caldwell, Uwe Braun and Thomas C Harrington (2006) Species of Cercospora associated with grey leaf spot of maize. Studies in Mycology 55: 189-197.
Verkley GJ, Quaedvlieg W, Shin HD and Crous PW (2013) A new approach to species delimitation in Septoria. Studies in Mycology 75: 213-305.
Quaedvlieg W, Verkley GJ, Shin HD, Barreto RW, Alfenas AC, Swart WJ, Groenewald JZ and Crous PW (2013) Sizing up Septoria. Studies in Mycology 75: 307-390.
Groenewald JZ, Nakashima C, Nishikawa J, Shin HD, Park JH, Jama AN, Groenewald M, Braun U and Crous PW (2013) Species concepts in Cercospora: spotting the weeds among the roses. Studies in Mycology 75: 115-170.
Groenewald M, Groenewald JZ and Crous PW (2005) Distinct species exist within the Cercospora apii morphotype. Phytopathology 95: 951-959.
Quaedvlieg W, Groenewald JZ, Jesús Yáñez-Morales M and Crous PW (2012) DNA barcoding of Mycosphaerella species of quarantine importance to Europe. Persoonia 29: 101-115.
Stukenbrock EH, Quaedvlieg W, Javan-Nikhah M, Zala M, Crous PW, (2012) Zymoseptoria ardabilia and Z. pseudotritici, two progenitor species of the septoria tritici leaf blotch fungus Z. tritici (synonym: Mycosphaerella graminicola). Mycologia 104: 1397-1407.
L Lombard, PW Crous, BD Wingfield and MJ Wingfield (2010) Phylogeny and systematics of the genus Calonectria. Studies in Mycology 66: 31-69.
WM Jaklitsch and H Voglmayr (2015) Biodiversity of Trichoderma (Hypocreaceae) in Southern Europe and Macaronesia. Studies in Mycology 80: 1-87.
Reference Strain
Astrosphaeriella aggregataMAFF 239485, MAFF 239486
Astrosphaeriella stellataMAFF 239487
Cercospora apiiIMI 077043, IMI 161116, MAFF 235978, MAFF 238072, MAFF 238299
Cercospora begoniaeMAFF 237690
Cercospora beticolaMAFF 238206, MAFF 305036
Cercospora capsiciMAFF 238227
Cercospora citrullinaMAFF 237872, MAFF 237913, MAFF 238205
Cercospora corchoriMAFF 238191
Cercospora ipomoeaeMAFF 239409
Cercospora kikuchiiMAFF 305039, MAFF 305040
Cercospora lactucae-sativaeMAFF 237719, MAFF 238209
Cercospora psophocarpicolaMAFF 305757
Cercospora richardiicolaMAFF 238210
Cercospora vignigenaMAFF 237635
Cercospora zinniaeMAFF 237718
Kalmusia scabrisporaJCM 12851, MAFF 239517, NBRC 106237
Katumotoa bambusicolaJCM 13131, MAFF 239641
Lasiodiplodia theobromaeMAFF 238880
Lecanosticta acicolaIMI 281598
Massarina arundinariaeMAFF 239461, NBRC 106238, NBRC 106239
Ophiosphaerella sasicolaJCM 13134, MAFF 239644
Phaeosphaeria brevisporaMAFF 239276, NBRC 106240
Phaeosphaeria sp.NBRC 106255
Polyplosphaeria fuscaJCM 13173, JCM 13175, JCM 13176, JCM 13177, MAFF 239683, MAFF 239685, MAFF 239686, MAFF 239687
Pseudotetraploa curviappendiculataJCM 12852, MAFF 239495, MAFF 239496, NBRC 106241
Pseudotetraploa javanicaJCM 12854, MAFF 239498
Pseudotetraploa longissimaJCM 12853, MAFF 239497
Quadricrura meridionalisNBRC 106242
Quadricrura septentrionalisNBRC 106243, NBRC 106244
Roussoella hysterioidesJCM 13126, MAFF 239636
Roussoella pustulansJCM 13127, MAFF 239637
Roussoella sp.NBRC 106245
Roussoellopsis sp.NBRC 106246
Roussoellopsis tosaensisJCM 13128, MAFF 239638
Sphaerulina myriadeaJCM 15565
Tetraploa sp.JCM 14424, NBRC 106251
Tetraplosphaeria nagasakiensisJCM 13168, MAFF 239678
Tetraplosphaeria sasicolaJCM 13167, MAFF 239677
Trichoderma aeroaquaticumNBRC 108031, NBRC 108034
Triplosphaeria cylindricaJCM 13169, JCM 14425, MAFF 239679, NBRC 106247
Triplosphaeria maximaJCM 13172, MAFF 239682
Triplosphaeria sp.NBRC 106248, NBRC 106249
Trisplophaeria acutaJCM 13171, MAFF 239681
Versicolorisporium triseptatumJCM 14775